Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.
Identifieur interne : 000426 ( Main/Exploration ); précédent : 000425; suivant : 000427Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.
Auteurs : Sheri Wang [États-Unis] ; Daibang Nie [États-Unis] ; J Peter Rubin [États-Unis] ; Lauren Kokai [États-Unis]Source :
- Lymphatic research and biology [ 1557-8585 ] ; 2017.
Abstract
Secondary lymphedema, resulting from damage to lymphatic vessels, is a common sequela following surgical removal of lymph nodes for cancer. Current therapeutics for treating lymphedema are limited and further research on underlying causes is warranted. Published studies on molecular mechanisms of lymphedema primarily focus on lymphatic endothelial cells (LECs), which comprise the innermost lining of lymphatic capillaries and collecting vessels. However, traditional static culture of LECs may not adequately recapitulate the lymphedemous cell phenotype as transcriptomal comparison of human dermal LECs has shown significant differences in ex vivo and in vitro LEC gene expression. In this study, we designed a dynamic culture system, in which LECs were exposed to physiologic and excess mechanical strain to determine if native and lymphedemous phenotypes could be reproduced in vitro.
DOI: 10.1089/lrb.2016.0042
PubMed: 28486010
Affiliations:
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sort="Kokai, Lauren" uniqKey="Kokai L" first="Lauren" last="Kokai">Lauren Kokai</name><affiliation wicri:level="2"><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh</wicri:cityArea></affiliation></author></analytic><series><title level="j">Lymphatic research and biology</title><idno type="eISSN">1557-8585</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Secondary lymphedema, resulting from damage to lymphatic vessels, is a common sequela following surgical removal of lymph nodes for cancer. Current therapeutics for treating lymphedema are limited and further research on underlying causes is warranted. Published studies on molecular mechanisms of lymphedema primarily focus on lymphatic endothelial cells (LECs), which comprise the innermost lining of lymphatic capillaries and collecting vessels. However, traditional static culture of LECs may not adequately recapitulate the lymphedemous cell phenotype as transcriptomal comparison of human dermal LECs has shown significant differences in ex vivo and in vitro LEC gene expression. In this study, we designed a dynamic culture system, in which LECs were exposed to physiologic and excess mechanical strain to determine if native and lymphedemous phenotypes could be reproduced in vitro.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Wang, Sheri" sort="Wang, Sheri" uniqKey="Wang S" first="Sheri" last="Wang">Sheri Wang</name></region><name sortKey="Kokai, Lauren" sort="Kokai, Lauren" uniqKey="Kokai L" first="Lauren" last="Kokai">Lauren Kokai</name><name sortKey="Nie, Daibang" sort="Nie, Daibang" uniqKey="Nie D" first="Daibang" last="Nie">Daibang Nie</name><name sortKey="Rubin, J Peter" sort="Rubin, J Peter" uniqKey="Rubin J" first="J Peter" last="Rubin">J Peter Rubin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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